Illumina ISeq 100 i1試劑卡盒V2 300個循環

Illumina ISeq 100 i1試劑卡盒V2 300個循環

Illumina 20009584

因美納

ISeq 100 i1試劑卡盒V2 300個循環

ISeq 100 i1Reagent Cartridge V2 300cycles

MADE IN USA

Illumina,20031371,TruSeq Index Plate Fixture Kit (2 fixtures)20009555/20009584

An iSeq v2 reagent cartridge (20031371, Illumina) is thawed in a water bath at room temperature for 1?h; the flow cell is inserted; and the kit is kept at room temperature. The LAMP-Seq library and the N-100 random-index library are diluted to 2?nM each in water. Next, 1?µl of the combined library is mixed with 150?µl of Tris pH 8.5. Then, 20?µl of the final dilution is loaded into the reagent cartridge and sequenced according to the manufacturer’s instruction using the following cycle numbers:

100 cycles read-1

8 cycles index-1

8 cycles index-2